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Copyright 1997 American Chemical Society). 5 s at 1 kV. Peaks: formamide (1), PTH-asparagine (2), PTH-glutamine (3), PTH-threonine (4), PTH-glycine (5), PTH-alanine (6), PTH-tyrosine (7) Capillary Electrochromatography: a Rapidly Emerging Separation Method 41 Obviously, the magnitude of the temperature effect on retention depends on the difference in the enthalpy of the solute in either phase, and is specific for each solute. Therefore, it also changes the column selectivity. There is no retention and no temperature effect for DH=0.

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S. Department of Energy under Contract No. DEAC03–76SF00098. 7 References 1. Steiner S, Witzmann FA (2000) Electrophoresis 21:2099 2. Rabilloud T (2000) Proteome research: two-dimensional gel electrophoresis and identification methods. Springer, Berlin Heidelberg New York 3. O’Farell PH (1975) J Biol Chem 250:4007 4. Gygi SP, Corthals GL, Zhang Y, Rochon Y, Aebersold R (2000) Proc Natl Acad Sci USA 97:9390 5. Wagner K, Racaityke K, Unger KK, Miliotis T, Edholm LE, Bischoff R, Varga GM (2000) J Chromatogr A 893:293 6.

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